Homologous recombination is useful in yeast genetics as an experimental tool to

Question

Homologous recombination is useful in yeast genetics as an experimental tool to alter chromosomal DNA. Which experiments in yeast would demonstrate that recombination was successful and that the desired gene was integrated into the yeast chromosome? A DNA molecule encoding the HIS gene is introduced into his haploid yeast to replace the mutant allele. Growth of the yeast in histidine-deficient media is measured. M ADNA molecule encoding the TRP gene is introduced into diploid yeast with a single trp mutation. Growth of the yeast in tryptophan-deficient media is measured ADNA molecule encoding a mutant allele of an essential gene is replaced by the wild-type allele in haploid yeast. Survival of the yeast is determined. A DNA molecule encoding a fluorescent marker within the ALTI gene is introduced into the yeast genome. Fluorescence is measured.

Explanation / Answer

A DNA molecule encoding a mutant allele of a n essential gene is replaced by the wild type allele in a haplois yeast. Survival of the yeast is determined

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