1. In studying receptor-ligand interactions, the researcher wanted to narrow dow
ID: 201185 • Letter: 1
Question
1. In studying receptor-ligand interactions, the researcher wanted to narrow down the exact region of binding between the receptor and the ligand. Amino acids 24- 29 on the receptor were believed to be involved in ligand binding. So the researcher performed mutations in these amino acids and assayed the effect of these mutations by performing binding assays. Mutations Receptor-Ligand binding Amino acid 24 No Amino acid 25 Yes Amino acid 26 No Yes Amino acid 27 Amino acid 28 Amino acid 29 Amino acids 24+27 Amino acids 24+26 Amino acids 25+27+29 No Yes Yes Yes No From these experiments.. What did the researcher learn from mutation in amino acid 26? (1 point) What did the researcher learn from mutation in amino acid 27? (1 Which of the amino acids are involved in receptor-ligand binding? Explain. (1 point) What do you conclude from the binding assay results of the two double mutation experiments? (1 point) What do you conclude from the binding assay results of the triple mutant? (1 point) If the amino acid 24 in question 3 was mutated from a glycine to an arginine, and the binding assay results were negative, what would you conclude from these results about the role of this amino acid in binding to the ligand? (1 point) 2. Explain in detail further. and also suggest other amino acid changes that would help understand these resultsExplanation / Answer
As 26 amino acid is changed hence no receptor binding was observed hence it suggest that this amino acid is crucial for binding. Due to mutation of this amino acid protein has lost its binding ability. As 27th amino acid alone does not affect the binding because here ligand and receptor binding is takes place. As individual amino acid level 24, 26 and 28 are involved in binding of proteins as single mutations results suggested. In double mutation 24+27 its showing it has binding that mean dual mutation may gain the ligan receptor binding. One more thing that can conclude from this 24th amino acid is crucial for interaction and mutation of 27th leads to take over the function of 24th amino acid. In second case hwere 24 and 26 both are important where mutations may leads to exchange their functions. Hence this double mutant will showing binding. In triple mutant we can conclude that all the three amino acid are performing same function. It is also possible that in single mutation two other residues balance the function of single residue; hence they may be similar or same amino acids. Once all the three amino acids mutated, hence, protein lost its function. Glycine is generally present in loop like structures and it is known to break the alpha helix and beta sheet structures. Whereas arginine makes the alpha helix hence it make constraint structure hence most of the other amino acids will not interact with cognate partner. Amino acid which generally break the alpha helix and beta sheet structures can promote the binding of the protein with ligand.
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