25 4 INTRODUCTION TO STAINING MICROORGANISMS QUESTIONS Completion 1. Microorgani

Question

25 4 INTRODUCTION TO STAINING MICROORGANISMS QUESTIONS Completion 1. Microorganisms must be 2. The main purpose of staining microorganisms is to before a basic-type dye will penetrate and stain them. stain enables you to 3. Both simple and differential stains dye the entire cell, but only a 4. If the organic part of a dye salt is positively charged, this dye should be called a(n) 5. The negative charge found on the surface of all particles, including microbial cells, is called the see what type of rod or sphere you are examining. dye, and if it is negatively charged, it should be called a(n) dye. This should have an attraction to -type dyes. 6. After a microorganism has been stained with a basic dye, where might the organic part of this dye salt be found associated with the cel? 7. Basic dyes stain (better or worse) as the pH of the culture medium is lowered (becomes more acidic) 8The organic ion portion of an acidic dye has a charge. 9. Complete the following sentence by adding the words increases or decreases: When a microbe releases acids into the the pH, growth medium, this the hydrogen-ion the acidity, and the numerical value for the negative log of the hydrogen-ion concentration. True- False (correct all false statements) 1. Simple stains enable you to determine the sizes and shapes of microbes and the type of rod or sphere you are examining 2. Both methylene blue and crystal violet are examples of acidic d 3. Simple stains color whole cells but only certain types of cells.

Explanation / Answer

1. Dehydrated

2. Visible under microscope.

3. Simple.

4. Cationic dye, anionic dye

5. Anions, anionic dye.

6. At the membraned organelles.

7. Better.

8. Positive

9. Decreases, increases, increases, increases.

Part - B

1. True.

2. False.

3. True.

Thanking you. Hope you got the answer.

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