SECTION QUESTIONS A-TESTING THE PURITY OF A COLONY Completion 1. A culture is co
ID: 201220 • Letter: S
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SECTION QUESTIONS A-TESTING THE PURITY OF A COLONY Completion 1. A culture is considered pure if (a) all cells taken from one colony type and st streaked on a plate show (b) cells from that colony examined with a light and (c) all subsequent streak microscope all appear plates and Gram-stained smears show these same characteristics. 2. A pure culture contains cells of one type. In other words, all cells are of the same True- False (correct all false statements) 1. Only colonies starting from a single cell are pure colonies. 2. Any colony beginning from more than one cell should be considered a mixed culture. 3. Difference in colony size is a good indicator of a mixed culture 4. Broth cultures should routinely be used to help determine whether a colony is a pure culture. 5. If a culture shows only short gram-negative rods, it should be considered a pure culture without need for further work- Multiple Choice (circle all that apply) 1. A mixed culture may be indicated by a. both gram-positive and gram-negative cells. b. various colored colonies. c. various sized colonies d. pulvinate and umbonate colonies having the same size and color. e. rhizoid-shaped colonies containing gram-positive rods and spheres. 2. You can be sure you have a pure culture if a. colonies contain only gram-negative rods. b. all colonies have the same color. . you d. all colonies are convex. e. the inoculum contained only gram-positive cocci. use cells from only one colony as an inoculum source.Explanation / Answer
1. Same size, shape and same color colonies.b) similar.
2. Same Species or same strain of microbe.
. True. Colonies from single cell are pure colonies.
2. True.
3. Same sized colonies in pure culture while different sized colonies are there in mixed culture. So, True.
4. Broth culture provides nutrients. So, False.
5.
1. Mixed culture contains both gram negative and gram positive cells, various sized colonies, various colored colonies.
2. You use cells from single colony then it must be a pure culture, all colonies have same color, contains only gram negative rods.
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