87 4. A researcher was measuring the absorbance of yellow food coloring. What wa

Question

87 4. A researcher was measuring the absorbance of yellow food coloring. What wavelength should he use on the spectrophotometer? Why? Food Coloring Food Casing 61 Red Food Culeing 02 01 (Taken from Orvis et al, 2007) 5. In your own words, explain how a standard curve is created and what purpose it serves. You used the hemocytometer to count algal cells, but found a very high concentration. To save time squares). You found 165 cells. What is the total concentration of cells in your sample (per ml)? 6. r of cells in ONE of the squares in the center (1 of the 25

Explanation / Answer

6. calculation for total cells

total cell/ml= total cell counted X dilution factor/ no of squares counted X 10,000

                      =165 X 1/1X 10,000 ( as the samples was not diluted, dilution factor is 1)

                      =165 X 10,000= 1.65 X 104 cells/ml

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