The Ames test identifies mutagenic chemical compounds by testing for increased r
ID: 201631 • Letter: T
Question
The Ames test identifies mutagenic chemical compounds by testing for increased reversion rates in auxotrophic bacteria exposed to a test compound in the pres liver In an experiment employing the methods of the Ames test, two his strains of Salmonella are used. Strain A contains a base substitution mutation, and Strain B contains a frameshift mutation. Four plates are prepared to test the mutagenicity of the compound ethyl methanesulfonate (EMS). Plate 1 is a control plate with Strain A and S9 extract but no EMS. Plate 2 is also a control plate and contains Strain B and S9 extract but no EMS. Plate 3 contains Strain A along with S9 extract and EMS, and Plate 4 contains Strain B, S9 extract, and EMS ence of detoxifying enzymes from the eukaryoticExplanation / Answer
Strain A : Base pair abstution
EMS do transition reactions by changing the single base.
Strain B: Frame shift mutation
Change in the frame due afdition or removal of one or more nucleotides.
option 1 is correct.
It is a part of minimal medium used for the growth of histidine deficit microorganism.
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