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nt.alt Geura/ : s it right, 3 Cirde al o the words that dexcribe this mediauo po

ID: 201975 • Letter: N

Question

nt.alt Geura/ : s it right, 3 Cirde al o the words that dexcribe this mediauo pointsJetfi er Enriched Differential Complex General Purpose Defined stio 4) Is it possible for a medium to be both defined and complex at the same time? Explain. (4 pdints) 5) s it possible for a medium to be both differential and selective at the same time? Explain. (4 points) A picture of the plate is below: 6) With which technique was the plate inoculated with bacteria (disregard the arrow in the picture)? (2 points) luak plade 7) The bacterium was determined to be E. coli, a common inhabitant in the intestine. This is a Gram negative, rod shaped bacteria with a flagella. Draw a picture of the cell, including the plasma membrane, cell wall (with important structures) and the flagella (with its 3 major parts). Label all structures. (6 points)

Explanation / Answer

Ans 4. No, it is not possible for a media to be defined and complex at the same time. There are certain bacteria that have specific requirements, thus they require defined media for their growth. Define media is also known as the synthetic media in which certain ingredients are present in specific amounts, and these type of media thus have specific recipes to prepare. This media is good for the fast growing bacteria.

On the other hand, in the complex media, the exact composition is not fixed. It is formed from the digested yeast, beef, soy and other additional proteins. This media is good for those microbes that are not too fast growing.

So. a media cannot be defined and complex at the same time.

Ans 5. No, a medium cannot be selective and differential at the same time. Differential medium contain specific ingredient plus a pH sensitive dye. So, when bacteria grows in such medium and produces its metabolites, these react having a specific pH, react with this pH sensitive dye and produce a color change.

However, the selective medium contains a special ingredient as required by each microbe to grow in a medium to grow.