C. Penetration of Alkali 1. Add 10 ml, of ieutral red solution to 15 ml. of yeas

Question

C. Penetration of Alkali 1. Add 10 ml, of ieutral red solution to 15 ml. of yeast suspensiou Take uote of auy chsuges in color that may occuu over a period of 5 min. Neutral red is red at plH below 7 but ye 2. Me low at plil between 7 and s asure 2 ml of the mixture and place in 4 labetled tubes. Add the folowing Test tube A 0.5 mL of 0.01N NH OH Test tube B 0.5ml of 0.01N KOH Test tube C 0.5mL of 0.01N NaoH Test tube D 0.5 mL of distilled water . Filter each mixture and observe the color of yeast cells and the fültrate under the nmicroscope

Explanation / Answer

ANS. For alkali cations the degree of hydration is probably is crucial in determining how far an anion penetrates.the degree of ionization is depends on of a chemical depends on its pKa and pH of the solutution.lithium the most strongly hydrated appeared not to penetrates as far as sodium and cesium. under certain conditions tantalum niobodium under go rapid penetration by liquid alkali metals along grain boundaries and certain crystalographic planes.penetration is observed when oxygen is dissolved in the refractory metal exceeds some threshould concentration.Alkaliesraises the tissue pH, causes saponification of fattyacids in cell membranes.there by resulting in disruption of the cell and stromal mucopolysaccharide with contamitant tissues softening. further penetration of Alkali in to the corneal stroma destroys the collagen fibers and matrix of the stromal matrix.

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