transcription/translation help 1) Write the sequence of t sequence directly belo

Question

transcription/translation help

1) Write the sequence of t sequence directly below) and the coding OF THE ELE strand that goes with the toflowing DNA template strand (wn the 2) Write the mRNA sequence that would result from transcription of the template strand. Cirele the start codon. 3) Translate the mRNA using the genetic code on acid sequence of the protein. the next page (Table 14.6 in your text). Write out the 4) Rewrite the DNA template sequence from first question, but remove the underlined base.

Explanation / Answer

1)Write the sequence of the coding strand that goes with the following DNA template strand (write the sequence directly below) and indicate which end is the 5’ and which is the 3’.

3’-TGCATTACGCCTAAATACGGGATTCCCGGAAAATTC-5’ (Template Strand)

5’-ACGTAATGCGGATTTATGCCCTAAGGGCCTTTTAAG-3’ (Coding Strand)

2) Write the mRNA sequence that would result from transcription of the template strand. Circle the start codon.

After Transcription both Sequences will get converted into mRNA strands.

5’-ACGTAATGCGGATTTATGCCCTAAGGGCCTTTTAAG-3’(Coding strand)

3’-UGCAUUACGCCUAAAUACGGGAUUCCCGGAAAAUUC-5(mRNA)

3’-TGCATTACGCCTAAATACGGGATTCCCGGAAAATTC-5’(template strand)

5’-ACGUAAUGCGGAUUUAUGCCCUAAGGGCCUUUUAAG-3’(mRNA)

3) Translate the mRNA using the genetic code on the next page (Table 14.6 in your text). Write out the amino acid sequence of the protein.

TRANSLATION Process

5’-ACG UAA UGC GGA UUU AUG CCC UAA GGG CCU UUU AAG-3’(mRNA)

5'-Thr   Stop Cys Gly   Phe   Met   Pro   Stop Gly    Pro   Phe    Lys-3'

3’-UGC AUU ACG CCU AAA UAC GGG AUU CCC GGA AAA UUC-5(mRNA)

4) Rewrite the DNA template sequence from first question, but remove the underlined base.

3’-TGCATTACGCCTAAATACGGATTCCCGGAAAATTC-5’ (Template Strand)

5’-ACGTAATGCGGATTTATGCCTAAGGGCCTTTTAAG-3’ (Coding Strand)

5) Write the mRNA sequence that will result from this mutated (base missing) DNA sequence in Question 4.

5’-ACG UAA UGC GGA UUU AUG CCU AAG GGC CUU UUA AG-3’(mRNA)

6) Write the translation of this mutant mRNA

5’-Thr   Stop Cys    Gly   Phe   Met   Pro Lys     Gly   Leu   Phe-3’

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