Escherichia coli: Positive or Negative? Pseudomonas aeruginosa: Positive or Nega

Question

Escherichia coli: Positive or Negative? Pseudomonas aeruginosa: Positive or Negative? Staphylococcus aureus: Positive or Negative? Il. Catalase activity: Purpose: To detect the presence of catalase, an enzyme During aerobic respiration, reactive oxygen species sucha formed. These are reactive intermediates that can damage D also alter the enzyme activity. If a bacterium tolerates or uses break those reactive oxygen species. One of those is catalas the enzyme catalase by simply adding H202 to a bacterial gro enzyme it will break the H202 and H2O and O2 will appear in t superoxide dismutase 202" + 2H+ 02 + H2O2

Explanation / Answer

Oxidase test

Oxidase test is carried out to identify bacteria, which produce cytochrome C oxidase and thus their electron transport chain is identified. Generally the bacteria which are oxidase positive are aerobic bacteria because because they have oxygen in their electron transport chain as terminal electron acceptor.

Out of the three given bacteria , oxidase positive bacteria are Pseudomonas aeruginosa and Staphylococcus aureus, while Escherichia coli is oxidase negative

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