Several temperature-sensitive mutant strains of E coli are shown to exhibit the

Question

Several temperature-sensitive mutant strains of E coli are shown to exhibit the following characteristics. Based on your knowledge of DNA replication components, predict what enzyme(s) might be affected by each mutation. (Note: answers can be used more than once AND multiple answers may be used for each question)

2. No initiation occurs

3. Synthesis is very slow

4. Okazaki fragments accumulate and DNA synthesis is never completed

5. Newly synthesized DNA contains many mismatched bases

6. Supercoiled and entangled strands remain after replication which is never complete.

a. DNA polymerase III

b. Replication clamp

c. Topoisomerase II

d. Ligase

e. DNA Polymerase I

f. DnaB

g. RNase H

h. DnaA

Explanation / Answer

The enzyme effected by DNA replication by each mutation are:-

2.Primase(RNA Polymerase enzyme)

3.DNA polymerases,DNA helicases

4.DNA ligase & DNA Polymerase

5.DNA Helicase

6.Topoisomerase

a.DNA Polymerase 3(Pol 3)

b.DNA Polymerase 3 holoenzyme

c.DNA gyrase

d.DNA ligase

e.Polymerase 1

f.dnaB helicase

g.Ribonuclease H

h.Helicase

b.

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