(3] The restriction enzyme EcoRI cuts the sequence GAATTC. Part of the coding se

Question

(3] The restriction enzyme EcoRI cuts the sequence GAATTC. Part of the coding sequence of a certain gene reads as follows: CAA AAC CTC AAG TCA ACG AGT TCG GTA ACG TAC Gln Asn Leu Lys Ser Thr Ser Ser Val Thr Tyr This part of the gene is PCR amplified from DNA of a patient with a disease that is often caused by mutations in this gene. It is noted that the PCR product, which in normal people is not cut by EcoRI, is now cut into two fragments. Assuming the mutation changes a single nucleotide in the segment shown, identify it.

Explanation / Answer

The CTC codon of Leu will be changed to "CTT" . This mutation allows EcoRI to cut. Because after this mutation EcoRI gets 3'CTTAAG5' sequences.

Related Questions

Navigate

• Browse (All)
• Browse #
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.