Prsiab Activlty 2 A Review of Electrophoresis Agause gel electrophoresis is a pr

Question

Prsiab Activlty 2 A Review of Electrophoresis Agause gel electrophoresis is a procedure used to sepearate DNA fragments based orn heir sizes ONA is an acid and has many negative electrical charges. Scientists have used bis fact to design a method that can be used to separate pieces of DNA. A solution containing a mibturs of DNA fragments of variable sizes is placed into a small well formed in an agarose gel trat has a texture similar to gelatin. An electric current causes the negatively-charged DNA molecules to move towards the positive electrode. Imagine the gel as a strainer with tiny pores that allow small particles to move through it very auickly. The larger the size of the particles, however, the slower they are strained through the gel. Aftar a period of exposure to the electrical current, the DNA fragments will sort themselves out by size. Fragments that are the same size will tend to move together through the gel and form bands. A piece of DNA is cut into four fragments as shown in the diagram. A solution containing the four fragments is placed in a well in an agarose gel. Using the information given above, draw (to the right) how you think the fragments might be separated. Label each fragment with ts corresponding letter A B C Pocitive Agarosn ge Have your teacher check your diagram before you proceed. 1. Where would the larger fragments, those with the greater number of base pairs, be ocated, toward the top of the gel or the bottom? Why? 2. Suppose you had 500 pleces of eech of the four fragments, how would the gel appear? 3. Itf t were possaible to weigh each of the fragments, which one would be the heaviest? Why? 4. Conplete this nude for the movement of DNA fragments through an agarose gel The lorger the DNA fragment, the

Explanation / Answer

1 answer: The larger piece of fragments present at the top of the gel , reason is due to its large size it becomes more difficult to be drawn through the gel.

2) Still there would be only 4 bands present.

3) Fragment D is the largest DNA piece, thus it would be heaviest and have the larger mass.

4) The larger the DNA fragment, the slower it migrates through an Agarose gel.

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