An important feature df palindrome and cuts the DNA only at that specific sequen

Question

An important feature df palindrome and cuts the DNA only at that specific sequence of bases A palindromic sequence can ba m 3 number of tirres on a strand of DNA, and te spechc restitin erzyne will cut all those palindromes, no matter what species the DNA comes from restriction enzymes is that each enzyme onty recognizes a spedific 8. If the GAATTC palindrome s repeated four times on the same piece of linear DNA and the restriction DNA, how many DNA fragments will be produced? enzyme that recognizes that base sequence is present and digests the 9 lf the GAATTC palindrome repeats are randomly found along the DNA strand, ten what can you say about the sizes of the fragments that will be produced when the DNA is digested with a restriction enzyme that recognizes that sequence? The table below shows palindromic sequences that are recognized by the ernzymes that are used to digest the DNA you will be analyzing in this activity. Name of restriction enzyme that recognizes the palindrome EcoRi GAATTC CTTAAG Hindill AAGCTT TTCGAA Pst CTGCAG GACGTO

Explanation / Answer

ANSWER 8

If palindromic site is present four times in a linear DNA then restiction enzyme makes four cuts in the DNA (one at each palindromic site) and five fragments of DNA are obtained.

ANSWER 9

We can't say anything about the sizes of DNA fragments obtained by digestion of DNA by restriction enzyme because we don't know the distance between the palindromic sites. The number of palindromic sites and distance between the palindromic sites will determine the size of fragments obtained.

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