8. Double stranded DNA will convert to single-stranded DNA if heated high enough

Question

8. Double stranded DNA will convert to single-stranded DNA if heated high enough; this temperature is known as the "DNA melting point:" Which DNA sequence would you expect to have a higher DNA melting point, one with 60% GC or one with 45% GC? Both are the same number of base pairs long. Explain your answer based upon the number of hydrogen bonds a GC base pair can form (versus an AT base pair). 9. Diagram the respective stages of the mitotic or meiotic cell cycle of a cell that has a diploid (2N) number of six chromosomes. Use different colored pens or pencils. Indicate some crossing over events. MITOSIS prophase MELOSIS prophase I metaphase metaphase I anaphase II anaphase anaphase I 28

Explanation / Answer

8. Ans- 60% GC

Because there is a significant effect of GC percentage on its melting point. G-C has three pairs of hydrogen bonds while A-T has 2 pairs of hydrogen bonds. The higher the percentage of GC, then it requires a higher temperature to break the bonds as compared to A and T, means higher melting point.

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