3. Now put a second red dot on the best fit line that is at exactly the double t

Question

3. Now put a second red dot on the best fit line that is at exactly the double the OD of the first do Record the time and OD f he number of cells must have doubled. Therefore, the time it took to go from the first OD reading to he second OD reading is the time it took for the culture to double. That is one generation time!!! t. or that second point. Since the OD double from the first to the second point, Use the two pairs of numbers you have marked to calculate the generation time for the culture you grew. (Different cultures may give different generation times.) OD at 1st red dot ,414 279 (30minutes Time at 1st red dot 15 minutes s minutcs OD at 2nd red dot Time 2nd at red dot Generation time 3. If you are told that a culture has an OD reading of 0.03 when there are one million cells per ml, how many cells per ml are present when the OD reads 0.015? 4. For the culture in question 3, what should you expect to get as an OD reading on a sample with six million cells/ml? 5. If you test a sample and find out that one million cells/ml of Escherichia coli has an OD reading of 0.23, would you expect the same OD, a higher or a lower OD if you had one million fungal cells? Explain your answer.

Explanation / Answer

3- OD represent the number of cells present in the culture. It is said that 1 OD equals to 8 *108 cells per ml.

Cells number increase exponentially as the OD increase.

So it 0.03 OD has 1 million cells then the 0.015 OD will have 0.5million cells.

5-

normally 1 OD of E-coli cells are around 8*108 cells

Yeast cells are bigger than the Ecoli cells so number of cells per OD of yeast is lesser than that of E-coli

1 OD of yeast cells are around 2*107 cells

So for One million Yeast cells will show higher OD than Ecoli's one million cells, as the number of cells in yeast is less as compared to E-coli

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