The following statements describe SDS-PAGE, a method used to separate proteins.

Question

The following statements describe SDS-PAGE, a method used to separate proteins. Select the true statements. Assume that SDS-PAGE is performed under reducing conditions. Smaller proteins migrate faster through the polyacrylamide gel. Protein-SDS complexes migrate toward the positive electrode. SDS-PAGE utilizes agarose gel to separate proteins. Proteins are visualized using a dye that binds to the gel matrix, but not to proteins. Sodium dodecyl sulfate binds proteins, resulting in protein-SDS complexes that are similar in size. Protein-SDS complexes have similar mass to charge ratios, therefore separation is by size.

Explanation / Answer

True statement on SDS PAGE

Smaller proteins migrate faster through the polyacrylamide gel

Protein-SDS complexes migrate toward the positive electrode

Protein-SDS complexes have similar mass to charge ratios, therefore separation is by size

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