Function Protein name Size based on pl based on amino acid amino acid sequence (
ID: 203704 • Letter: F
Question
Function Protein name Size based on pl based on amino acid amino acid sequence (in sequence kDa) Pro1P Flx1p 215 210 7.5 7.3 Binds to AMP and Ca Protein kinase, catalyzes the reaction between a protein and ATP to give ADP and a phosphorylated protein Binds to iron (Fe) Protein phosphatase, catalyzes the reaction that removes a phosphate from a phosphorylated protein Unknown but does bind to Zinc metal ions Hmm1p Cnc1p 120 35 5.4 8.8 Unk1p 42 7.8 4.D 4a. You ran these proteins through an isoelectric focusing gel over a pH range of 6.0 to 85 Draw out what you would expect to see for the proteins separated through this isoelectric focusing gel. 4b. What if when you ran the isoelectric focusing gel over a pH range of 6.0 to 85 and you found that there were two protein bands corresponding to k2p. What most likely explains the existence of two protein bands here? FLX1P 4c. What could you do to confirm that your explanation from 4b above is correct?Explanation / Answer
4A) In isoelectric focusing, you have a strip that has a gradient of pH (ex 6 to 9) and is properly labellled about where each pH value lies in the strip. So, after iso electric focusing bands would be observed on the strip at the location corresponding to the PI of the protein.
So, a band should be observed at 7.3, 7.5, 7.8 and 8.8 ( as they all falls between the range). Band for PI 5.4 would not be observed in strip of this range.
4B) The most likely reason might be that the FLx1p and Pro1p are having a very close PI so spacing between them might not be clearly visible.
4C) you can confirm this by runing a 2 dimentional gel eleectrophoresis of the strip, you should be able to seperate the two proteins on basis of molecular weight now.
These answers are to the best of my knowledge. You can leave a comment down below in case you have any further query. Thank you.
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