What will happen to the expression of the tryptophan operon of E. coli when tryp

Question

What will happen to the expression of the tryptophan operon of E. coli when tryptophan is removed from the following cultures of mutant strains that are growing in the presence of tryptophan? Note: Assume that attenuation accounts for a ten-fold regulation and that the trp repressor proteirn accounts for about a 70 fold regulation. a) The tryptophan repressor protein is mutant so that it cannot bind tryptophan b) The tryptophan repressor is mutant so that it cannot bind the trp operator. c The start codon for the leader peptide is mutant. d) The series of T-A base pairs at the end of the attenuator region is deleted.

Explanation / Answer

Ans. The correct option ib option B.The tryptophan repressor is mutant so that it cannot bind the the trp operator. Due to the unavailability of tryptophan the trp repressor is inactive it doesnt attach to the DNA or block transcription which results in trp operon to be transcribed by RNA polymerase.

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