You have isolated Protein X with a known function Y. Based on sequencing; you be

Question

You have isolated Protein X with a known function Y. Based on sequencing; you believe that it is a double pass transmembrane protein at the ER. What can you do experimentally to this protein to test whether or not it is a double pass membrane protein while still ensuring that it remains in the ER membrane? Include the following components in your answer.

Hypothesis: Protein X is a double pass transmembrane protein with function Y

Experimental Control:

Experimental Group:

Method(s):

Result/Analysis:

How could you verify that it is still in the ER membrane?

Explanation / Answer

if you have the sequence of the peptide, you can synthesize a synthetic peptide based on the exact same sequence. You can perform hydropathy plot. A hydropathy plot will give you two regions with hydrophobic amino acid residues if the ER membrane is a double pass transmembrane protein

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