Question 10 1 pts Pre-assessment. Your control plas id has a concentration of 8

Question

Question 10 1 pts Pre-assessment. Your control plas id has a concentration of 8 ng/m You add 1 1 of plas id DNA to 75 of competent cells and incubate on ce for 30 minutes. You a minutes. You the perform three serial 10 fold dilutions and plate 00 of the last dilulti , onto an B + AMP plate. The next day there are 80 o ies on the te. what this information? 925 of roth. ndal o the a sfo mation cer the ceils recover r45 e com petent e based on 2 x 10° CFU/ 1x 10 CrU/HS 2 x 107 CFU/ O 1x1010 CFU/H 1x 106 CFUg

Explanation / Answer

D) 1 x 1010 cfu/µg

Colonies = 80
µg DNA = 8 X 105
Dilution = 103 and then 100 µl from 1 ml means 104
TE = 80 / 8 X 105 / 104 = 1 x 1010 cfu/µg

So, the transformation efficiency is 1 x 1010 cfu/µg

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