4i) Suppose that you made a right-side out vesicle and sealed it around one set

Question

4i) Suppose that you made a right-side out vesicle and sealed it around one set of substances (see ta ble) and then surrounded it with another combination (see table). In each case the appropriate counter ions would be present to initially result in electrical neutrality and here would be the appropriate osmolytes to prevent osmotic changes in the RBC volume. Using the standard model for the Na/K pump determine if you would expect there to be ATPase activity? Na pumping? K pumping? Fill in columns 4-6 with the information. Note: to result in a measurable amount of pumping or ATP hydrolysis, the pump would have to undergo more than one cycle

Explanation / Answer

ATPase Na+ pumping k+ pumping Any ATPase A N N N Y B Y Y Y Y C Y N N Y D N N N N E N N Y Y F Y N N Y G Y Y N Y H Y N N Y I Y    N Y Y J Y Y N Y

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