Namé: ID: A 25. Which of the following treatments will work to extract and isola

Question

Namé: ID: A 25. Which of the following treatments will work to extract and isolate chromosomal DNA from bacterial cells? a. Add lysozyme to cells in buffered solution, treat with detergent and enzymes to remove proteins, centrifuge and then collect DNA in the supernantant b. Add enzyme cocktail containing lysozyme, RNase, and proteases to burst cells, then salts and detergent to remove proteins, followed by centrifugation, where supernanatant is dumped and the pellet is resuspended in buffer to collect DNA e. Add NaOH and SDS to burst cells, precipitate out proteins, and isolate DNA through a series of ethanol and isopropanol precipitations d. Centrifuge cell mixture to burst cells, collect supernatant, precipitate out peoteins with TE buffer, centrifuge again and add lysozyme 26. After the gene for a protein of interest has been identified, there are several methods for locating it. Which of the following is not one of those methods? a. Fluorescent DNA probes can be used to hybridize to the DNA sequence of interest and be detected. Protein assays can be performed. DNA fragments can be electrophoresed through a gel and the DNA fragment of interest can be detected by Southern blotting. PCR products can be generated with the use of primers and a thermal cycler. b. c. d. What does it mean to probe DNA? a. b. c. d. Which restriction enzyme listed in the chart below will result in a blunt end cut? 27. Performing an ethanol precipitation and then spooling the DNA with a glass rod Locating a gene of interest using a short complementary piece of DNA Treat with restriction enzymes and ligate the sticky ends Spin the DNA in a centrifuge and collect the supernatant 28. Restriction Enzyme Alu I Eco RI Hindlll AG CT GAAATTC A AGCTT a. Alu I b. Bam HI c. Hindll! d. All of the above After cell lysis, the cell contents are removed from solution, leaving DNA, by using all the reagents liste except: a. RNA b. Solvents c. Centrifugation d. Enzymes How is DNA spliced? 29. 30. a. PCR b. Scalpel e. Restriction enzymes d. Probes

Explanation / Answer

25. Option b is correct for the extraction and isolation of DNA.

26. Protein assay cannot be one of the methods to locate DNA but to identify the protein.

27. DNA probe is utilised in locating a gene of interest using a short complementary strand of DNA.

28. Alu I enzyme produce blunt ends i.e. straight cut.

29. RNA is not utilised for the DNA extraction.

30. DNA spliced by restriction enzymes.

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