3. You are testing the effect of adding arabinose and glucose to growth media on

Question

3. You are testing the effect of adding arabinose and glucose to growth media on induction of GFP expressed in the pGLO plasmid by E. coli, as we did in Lab 2 The following chart shows the conditions used Tube l Volume of 20% arabinose Volume of 20% glucose 40 40 40 40 20 40 5 a. What is the concentration of arabinose and/or glucose in each tube? 5 b. You performed a Bradford assay as described in Lab 3. The chart summarizes your results: 30 uL of Average O 0.23 0.58 1.00 0.92 0.90 0.86 0.76 0.83 BSA [0.2 mg/mL] BSA [0.5 mg/ml 1/4 2/4 3/4 4/4 5/4 Calculate the protein concentration in each sample c. Determine how much of each sample and how much buffer to add to each of 5 tubes, so that the same of amount of total protein is in each tube, in a volume of 30 ul

Explanation / Answer

20% means 20ug/1ul

40ul=800ugs, 20ul=400ugs

The protein concentrations are0.82, 0.8, 0.77, 0.65, 0.79 ugs respectively by intercepting on the graph

6uls of protein sample in each test tube and make it to one ml with water..

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