16. Select the false statement about SDS-PAGE performed under a. Smaller protein

Question

16. Select the false statement about SDS-PAGE performed under a. Smaller proteins migrate faster through the polyacrylamide & b. Proteins are separated in a polyacrylamide gel matrix c. SDS binds proteins, resulting in protein-SDS complexes t Pump d. tein-SDS complexes migrate toward the negative electrode e. Proteins are visualized using a dye that binds to the pro Answer the following three questions based on the figure on the rgnt 17. The method on the right is called and is used for a. ion exchange chromatography protein separation b. size exclusion chromatography ; protein separation c. affinity chromatography: protein sequencing d. size exclusion chromatography ; DNA sequencing e. isoelectric focusing determination of pl 18. In this method, the last. a. smallest; largestmolecules will elute first, and the b. positively charged ; negatively charged c. most highly charged ; least highly charged d. highest pl; lowest pl e. largest; smallest 19. Among the followin this method? g list of proteins, which pairs would you be able to separate using 1: Porous polymer beads monomer of 66 kDa ; pl 4.5; strong affinity for carbohydrates 2: tetramer of 16 kDa monomers, pl - 8.0; strong affinity for DNA 3: monomer of 18 kDa ; pl = 4.6 ; strong affinity for carbohydrates a. 1 and 2 c. 2 and 3 b. 1 and 3 d. both 1 and 2 and 1 and 3 e. none

Explanation / Answer

16. Option D is correct.
SDS is an anionic detergent. It imparts a negative charge to proteins. So, they migrate towards the positive electrode.

17. Option B is correct.
The given image represents a size exclusion chromatography column used to separate proteins.

18. Option E is correct.
In size exclusion column, largest molecules are eluted first and the smallest molecules last.

19. Option D is correct.
Proteins 1 and 2, 1 and 3 significantly differ in their sizes. So, they can be separated using size exclusion column.

Related Questions

Navigate

• Browse (All)
• Browse 1
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.