ookmarks People Window Help Home Insert Design Layout References CH.7, CH, 19 Me

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ookmarks People Window Help Home Insert Design Layout References CH.7, CH, 19 Membranes/Enc × e.com/courses/32813/quizzes/26773/take/questions/356703 s freezes or logs ABC you out for inactivity, you can log back in and resume. All exams must be submitted by 8:.40 PM at time, you will be able to review your scored exam by clicking on Grades and on the exam. Good luck Question 19 4 pts A graduate student working in my lab wishes to target a plasma membrane protein to the peroxsomes of oails ih cuturs Wha experiment would be necessary to accomplish this task? The student would need to clone the codons for a start-transter sequence into the genc. The student would need to clone the codons for a stop-transfer sequence into the gene The student would need to clone in an M6P tag. The student would need to mutate and cause a defecet n th syune iue the signul Bogpnibion cells sequence into the gone and delete the ER signal sequence The student would need to clone an SKL Previous 0

Explanation / Answer

Protein which needs to be designed for peroxisome needs to have a signal sequence Ser-Lys-Leu or S-K-L at their C-terminal. This SKL sequence helps in binding the protein to a cytoplasmic soluble protein  PTS1R. The complex of  PTS1R-protein is then recognized by the receptor protein Pex14p, present on the membrane of the peroxisome which helps in the entry of protein in the peroxisome.

So correct answer is option E.

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