each other by the tests done in this exercise? S agalactiae from S. pyogenes S.
ID: 205719 • Letter: E
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each other by the tests done in this exercise? S agalactiae from S. pyogenes S. pneumoniae from the other three streptococci Which two organisms are alpha hemolytic? What causes the arrowhead shape in the CAMP test? 3. 4. olated a Gram positive, coccus-shaped bacterium from a sick patient. What enzyme could u test for to determine if the isolate is Streptococcus or Staphylococcus. Using the tests for hemolysis and sensitivity to antibiotics, how would you determine thata Streptococcus sp. you isolated from a sick patient was S. pyogenes and not S pneumoniae, S. mutans or S agalactiae? 6.Explanation / Answer
2. The CAMP test helps distinguish S. agalactiae from S. pyogenes as they can produce a diffuse extracellular compound called CAMP substance. This in conjunction with S.aureus produces increasead hemolysis of blood cells.
S. pneumoniae are distinguished from other streptococci by the fact they are alpha hemolytic and are bile solube and inhibited by optochin
3. S. pneumoniae and S. viridans are alpha hemolytic.
4. Arrowhead shape is caused by enhanced hemolysis which is caused by conjunction of the two organisms S. agalactaciae and S. aureus.
5. Catalase test can be done to distinguish between Staph and Strep as Staph are catalase positive while strep are catalase negative.
6. The Lancefield antigen A test along with bacitracin test can be used to distinguish S. pyogenes from other streptococci as S.pyogenes is sensitive to bacitracin and other strep are negative to this. Even if Lancefield antigen A test may be positive in certain group of streptococci a definite sensitivity to bacitracin has been a classic feature with S pyogenes.
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