You are working in a clinical lab and a fellow technician has recorded the resul

Question

You are working in a clinical lab and a fellow technician has recorded the results she obtained for the butts and slants of inoculated KIA slants as follows: 7. Slant A A IAlk /H2S neg/CO2 neg Alk /A /H2S pos /CO2 pos Slant B Unfortunately, she failed to specify in what order (butt or slant) she listed the results. Because of your background in microbiology, you know that the and th because can be fermented only if the bacterium can ferment e result is for the slant in both Slant A and Slant B. You know this result is for the 8. Continued from Question 7: Slant A will have abutt and agar. Slant B will have a butt and a bubbles in the bubbles in the agar. slant a slant

Explanation / Answer

KIA slant interpretation -

A/ALK result is for the Butt and slant respectively for Slant A. and

ALK/A result is for the slant and butt respectively for Slant B.

sucrose can be fermented only if the bacterium can ferment lactose.

Slant A red slant and yellow butt with no gas production and

Slant B yellow slant and black butt with CO2 bubbles in agar.

Butt / slant interpretation yellow/yellow glucose, lactose or sucrose are fermented yellow/orange-red glucose only fermented orange-red/yellow acid prod.,glucose utilized aerobic orange-red/orange-red no fermentation of sugar bubbles/uplift/cracks gas production black hydrogen sulfide production

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