A graduate student is studying liver cells in a rare green striped hamster. He a

Question

A graduate student is studying liver cells in a rare green striped hamster. He adds radiolabeled Cis acyl-CoA but no radiolabeled trans-A2-Enoyl-CoA is detected. Additional experiments indicate that both FAD and the electron transferring flavoprotein are structurall y intact. In a separate experiment radiolabeled succinate is added and no radiolabeled fumarate is detected. Succinate dehydrogenase is found to be structurally intact. Upon further analysis of these cells, the electrochemical potential across the inner membrane of the mitochondria was found to be extremely low. In a final experiment radiolabeled glycerol-3-phosphate was added to the cells but no radiolabeled dihydroxyacetone phosphate was detected. Glycerol 3-phosphate dehydrogenase was found to be structurally intact. What is wrong with this poor hamster? Explain all of these results. Design an experiment to test your hypothesis.

Explanation / Answer

Ans.Even if radiolabelled acyl coA no radiolabelled trans enoyl coA is formed this can be because of different fate of acyl coA in liver.If the animal is starving then acyl CoA will be diverted to another pathway to produce sugar.So test to calculate blood sugar can be done, another situation can be if there no carnitine catalyst present then acyl CoA cannot enter mitochondrial inner membrane for b oxidation of fatty acid.

All the pathways are interconneted.If the animal is starving then glucose is required in more amount so there is less chance that glycolysis will take place.if there is no glycolysis then citric acid cycle also will not be initiated.So we can say that poor hamster is starving

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