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Can you help me do 3, 4? Thanks. 3. The Microbiology Lab performed the following

ID: 206232 • Letter: C

Question

Can you help me do 3, 4? Thanks.

3. The Microbiology Lab performed the following blochemical tests on isolated colonles plates. The results from the tests are listed below: Nutrient broth grown at 35oC- turbid Nutrient broth grown at 4oC-not turbld Oxidase test-colony turns black Nitrate test-broth is turbid and turned red after addition of reagents to test for reduction of nitrate Phenol red Glucose fermentation broth-turbidity and yellow color, no bubble in durham tube Phenol red Lactose fermentation broth-turbidity and red color, no bubble in dirham tube Phenol red sucrose fermentation broth-turbidity and turned yellow, no gas in durham tube VP test-broth is turbid and has red color at the top after adding reagents 4. Using the biochemical tests in #3, what organism (Genus and species) is causing the infection?

Explanation / Answer

Serratia marcescens is the organism causing infection

Biochemical characteristics of Serratia marcescens

Oxidase test: Negative

Nitrate test: Positive

VP test: Positive

Glucose fermentation: Positive fermentation but no gas formation

Lactose fermentation: Negative

Sucrose fermentation: Positive fermentation but no gas formation

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