Transcription 1. Below is DNA molecule. The arrow shows the location and directi

Question

Transcription 1. Below is DNA molecule. The arrow shows the location and direction of the promoter 3" AT GATCTCGTAA 5' 5' 3' DNA 2. Which enzyme is responsible to synthesizing new mRNA nitrogenous bases? (1 pt) a. Which direction does it read" the parent DNA strand? 5to3'(1 pt) b. Which direction does it synthesize the nitrogenous bases? to(pt) Based on the direction on the promoter, which DNA strand will be transcribed? The upper or the lower strand? 3. (1 pt) 4. Transcribe the appropriate strand and write the mRNA transcript below (just listing the s base letters is sufficient). Make sure to indicate the 5' and 3 ends (3 pts) 5. How many codons comprise the mRNA transcript? (1 pt) Translation 6. mRNA translated in the to--, direction. (1 pt) 7. During the translation of mRNA, how many tRNA molecules will bind to the ribosome? (2 pts) a. For each tRNA, list their anticodon in the space below (2 pts) Translate the mRNA transcript(in #4) into their produced amino acids. Write the ami acids in the correct order in the space below (2 pts). 8,

Explanation / Answer

2) Enzyme responsible to synthesize new mRNA nitrogenous bases is RNA polymerase.

2.a) In transcription RNA molecule that synthesized is always complementary & antiparallel to DNA template strand. Also transcription just like DNA replication is always takes place in 5' to 3' direction. So, in order to complementarity it will read the parent DNA strand in 3' to 5' direction.

2.b) From the above explanation it is clear that it will synthesize nitrogenous bases from 5' to 3' direction.

3) From the explanation of the previous question it's clear that DNA template required to be in 3' to 5' direction. So the Upper strand will be transcribed.

4) mRNA transcript- (As it is RNA so here Uracil (U) will be added in place of T)

                                           5'-UACUAGAGCAUU-3'

5) Codon is a sequence of three nucleotides (Eg. First 3 nucleotides UAC is a codon. Similarly UAG, AGC & AUU is also codon in above example). Here 12 nucleotides are found. So there will be 12/3= 4 codons.

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