1. Fill out the following table by indicating which general technique (light mic
ID: 206721 • Letter: 1
Question
1. Fill out the following table by indicating which general technique (light microscopy [LM] or electron microscopy [EMI]) could be used to observe each structure or phenomenon. Put "no" in the box if the technique could not be used. If light microscopy can be used, name one technique (bright field, phase-contrast, fluorescence, etc.) that you think would be effective. Structure or phenomenonname one type/technique Could LM be used? If so, Could EM be used? If so, any specific staining/preparation? Motion of chloroplasts in live plant cells The structure of a corona virus Cell viability (alive or dead) Thylakoids in a plant cel Distribution of proteins in the plasma membrane of an animal cell. 2. Briefly state the function of each of the following parts of a light microscope. a) Field iris diaphragm: b) Condenser iris diaphragm: c) Condenser focus knob: d) Objective lens: e) Ocular lens: f Interpupilary adjustment:Explanation / Answer
motion of chloroplast in live plant cell - light microscopy can be used. phase contrast microscope. no EM.
structure of corona virus - no LM. EM can be used. staining uranyl acetate and phosphotungstic acid.
cell viability - phase contrast LM. no EM.
Thylakoid in a plant cell - no LM. EM can be used. staining uranyl acetate and phosphotungstic acid alos called as negative staining.
Distribution of a protein in the plasma membrane - fluorescence microscopy. no EM.
2) a) - controls the amount of light reaching to the specimen.
b) - controls anount and quantity of light reaching to the specimen.
c) - move the objective lenses toward or away from the specimen.
d) - to magnify the specimen and resolution.
e) - to enhance magnification and resolution of the specimen.
f) - twisting the eyepieces/ocular lens in a vertical arc. to achieve the proper interpupillary distance.
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