12. Two patients display the disease Hb Providence. The isoforms of Hb Providenc
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12. Two patients display the disease Hb Providence. The isoforms of Hb Providence involve substitution mutations that alter the charge residues on the protein. A DEAE-ion exchange column is provided to separate a mixture containing a normal and 2 isoforms of Hb Providence. 1) Describe the experimental parameters you might use to separate the normal from the mutant proteins, 2) explain the premise for choosing the experimental parameter of your choice and 3) provide an hypothetical graph illustrating the elution profile of the proteins. (10 points)Explanation / Answer
(a)The 2 isoforms of Hb are HbA & HbB can separated gel electrophoresis & chromatography (affinity chromatography)
(b) the presence of charge on the mutant proteins particles i.e. polarity of particles; is main premise for choosing the gel electrophoresis & chromatography
(c)the images can not be uploaded
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