2. Write the chemical reaction that occurs in a gas pak jar to create an anaerob

Question

2. Write the chemical reaction that occurs in a gas pak jar to create an anaerobic environment. 3. An organism that grows as a facultative anaerobe in thioglycollate is inoculated on three diff erent nutrient agar plates. One plate is incubated in atmospheric oxygen, another with a gas pak, and the third in a candle jar, what do you expect the results to look like for each plate? Sketch and describe/explain your answer. 4. Repeat question 3 for a strict aerobe, a microaerophile, and a strict anaerobe. 5. Where in/on the body might anaerobic or facultative anaerobic microbes grow? Explain. 6. Why was BHIA media used in addition to NA in this lab?

Explanation / Answer

2) Chemical reactions

NaBH4 + 2 H2O = NaBO2 + 4 H2

C3H5O(COOH)3 + 3 NaHCO3 + [CoCl2] = C3H5O(COONa)3 + 3 CO2 + 3 H2 + [CoCl2]

2 H2 + O2 + [Catalyst] = 2 H2O + [Catalyst]

The GasPak Anaerobic System is used to form an oxygen-free surroundings for the growth of anaerobic microorganisms.

Anaerobic conditions are created by adding water to a gas generator envelope, which is placed in the jar just before sealing. The envelope contains two chemical tablets, sodium borohydride (NaBH4) and sodium bicarbonate(NaHCO3). Water reacts with these chemicals and produces H2 gas(hydrogen gas) and CO2. The hydrogen gas combines with free oxygen in the chamber to produce water and removes all free oxygen from the chamber. This reaction is catalyzed by the element palladium, which is attached to the underside of the lid of the jar. The CO2 replaces the removed oxygen and creates a completely anaerobic surroundings.

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