9 A)You are trying to separate two blood proteins: transferrin (76 kDa) and lyso
ID: 206898 • Letter: 9
Question
9 A)You are trying to separate two blood proteins: transferrin (76 kDa) and lysozyme (15 kDa). Which of the following is correct?
Differential centrifugation is a useful method to separate these two proteins.
Transferrin will sediment more quickly during rate zonal centrifugation.
Transferrin will run through a gel filtration chromatography column more quickly.
Lysozyme will be found nearer the top of the gel if you run these proteins on SDS polyacrylamide gel electrophoresis.
9 B) You have isolated an antibody and would like to know more about the protein it recognizes. Which experiment will give you the LEAST information about this protein?
antibody-affinity chromatography to isolate the protein, followed by cryoelectron microscopy and/or NMR
Western blotting of extracts derived from biologically different samples to determine the levels of your protein of interest
rate zonal centrifugation followed by 2D gel electrophoresis
co-immunoprecipitation followed by mass spectrometry of binding partners
Explanation / Answer
9. Option C is correct.
In gel filtration chromatographic column, proteins with larger size are eluted first followed by proteins with low molecular weight.
Since transferrin has high MW, it will be eluted first from the gel filtration column.
10. Option D is correct.
Coimmunoprecipitation with the given antibody will precipitate all the proteins that can be recognized by the antibody. Mass spectrometry of this sample will reveal the identity of bound proteins.
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