3. (1 point) In an attempt to repeat Beadle and Tatum\'s seminal experiments wit

Question

3. (1 point) In an attempt to repeat Beadle and Tatum's seminal experiments with Neurospora, you have isolated four different mutants unable to synthesize some compoun for growth. You know that compounds A through D are required in the biosynthetic pathway, but you do not know the order in which they are synthesized. You test each of the following growth products for its ability to support the growth of each mutant. d E that is necessary supplement added Mutant 2 4 Using the data above, construct a biochemical pathway indicating the order of the compounds. For each mutant, indicate where in the pathway it is blocked. Explain how you determined your answer.

Explanation / Answer

In such type of problems first find which one is the final product or on which substrate all the mutant s is growing.

In this substrate E is the main product without that no strain will survive. Hence, supplementation it is growing.

Than find out how many intermediates compounds are forming.

Here here A,B,C and D are intermediate

Find out which one is maximum auxotrph mean while providing all the intermediate substrate it is not growing on any media, here mutant 1 is not growing any media hence the enzyme that convert substrate into final product is missing. Hence it is growing on when supplemented E.

Now second maximum auxotroph is mutant 4 but it convert intermediate B to E.

Now check third maximum auxotrophs is mutant 3 that grows on substrate intermediate B and C. so here C form B and B form E so order is

C---B--E

Similarly next intemediat is A.

A--C--B--E

Last is D

D-- A--C--B--E

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