1. Define diabetes Types I and II. How are they different? What are some possibl

Question

1. Define diabetes Types I and II. How are they different? What are some possible treatments? Find the answers by reading this web: http://www.nobelprize.org/educational/medicine/insulin/diabetes-insulin.html

2. Describe how insulin is made in bacteria. What was the source of the gene that was cloned? What antibiotic was used to select for cells that got transformed with the recombinant insulin plasmids? Find the answers by going to: http://www.dnalc.org/view/15928-How-insulin-is-made-using-bacteria.html and https://www.youtube.com/watch?v=Jfl2Ti4Ce8s

3. How is insulin made recombinantly in yeast? Compare and contrast the yeast process with the bacteria process. Find the answers here: http://www.dnalc.org/view/15929-How-insulin-is-made-using-yeast.html

4. Based on the below powerpoint, what type of chromatography was used to purify insulin? http://www.slideshare.net/sol777/design-and-purification-of-proteins

5. Describe how glucose monitor meter works by watching this link: https://www.youtube.com/watch?v=Tzc5QYHxfdU N

Explanation / Answer

1)type 1 Diabetes - it is a type of Diabetes which occurs approximately 10 percent cases. It is an autoimmune disease in which immune system bymistake attack it own insulin peoducing cells due to which insufficient amount of insulin is produced in the body which result in the formation of type 1 Diabetes. It generally occurs to young age people.

Type 2 Diabetes - it occurs in the 90 percent of all the cases of Diabetes. It generally affect the people during second half of their life. In this case insulin become resistance to the body and ineffective as well.

Possible Treatment in this as follow-

For the case of type 1 it can be treated easily with the help of insulin dose to the body but the type 2 is little bit complicated becasue it happens when the immune of body become very low. For this the Treatment are dylasis and insulin dose. Drug for insulin and daily physical ecercise and meditations.

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2)

Synthetic human insulin was the first golden molecule of the biotech industry and the direct result of recombinant DNA technology. Currently, Billions of diabetics worldwide use synthetic insulin to regulate their blood sugar levels in it. Synthetic insulin is made in both bacteria and yeast.

3) the insulin is prepared from yeast by the help of fragmentation of the yeast in such a biological conditions which favour the formation of insulin.

The formation of insulin formation from bacteria is more good as compared to produced it from yeast. It is because it is efficuent easy and can be produced in bulk amount.

4)cation Exchange chromatography is used in the purification of the insulin.

5)it work on the Principle of electromagnetical current foemation. In this meter there is a strip in which the test blood is placed and the strip is attached to the machine. This blood produce electro magnetic induced current with the device. After calibration we get the result.

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