REVIEW QUESTIONS 1. Of the media inoculated, EMB plates, HE plates, TSI slants a
ID: 207101 • Letter: R
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REVIEW QUESTIONS 1. Of the media inoculated, EMB plates, HE plates, TSI slants and Enterotube; which gave the most information about the biochemical characteristics of the organisms? 2. How can the 4 organisms be distinguished from each other using their reactions in the TSI slants? 3. A clinical specimen, such as a fecal or urine sample, should not be inoculated directly into an Enterotube. Why not? 4. Which Enterotube tests were incubated anaerobically? 5. Which component(s) of EMB medium are selective? Which are differential?Explanation / Answer
Hi,
The questions 1 and 2 cannot be answers as the names of the organisms are not mentioned. It must have been given in the book.
3. The enterotube is a rapid detection kit, which helps to identify the carbohydrate fermentation, oxidase - reductase and many more biochemical characters of the pure species. The clinical specimen like fecus or urine sample, contain different type of microbes with different biochemical properties. If these are directly added to the enterotube, we cannot differentiate and identify the type of organism present in the clinical sample. The inoculum must be a pure culture obtained from selective plates.
4. Anerobic compartments are: First 4 comprising of Glucose, Lysine, Ornithine and Indole.
5. Methylene blue and Eosin-Y are the selective components of the EMB agar. They inhibit the growth of gram-ve organisms.
The differential component is the lactose. It helps to differentiate between the lactose fermentors and non fermentors withing the enterobacteriaceae. The lactose fermentors form black colonies whereas the non fermentors form colorless colonies.
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