a suspension of purified mitochondria was prepared in a buffer at pH 6.7 and 25C

Question

a suspension of purified mitochondria was prepared in a buffer at pH 6.7 and 25C. The pH of the matrix was determined to be 7.3, and the electrical charge difference across the inner mitochondrial membrane (IMM) was 0.14V (inside negative). Suppose that one more of H+ ions flow through the F0F1-ATP synthase (embedded in the IMM). What is the total energy available for ATP synthesis? delta G'= ? kJ/mol What percentage of the total energy calculated above is associated with 1) the difference in pH across the IMM? ___% 2) the difference in the electrical charge across the IMM? ___%

Explanation / Answer

Estiamted consumption of the proton gradient by ATP synthesis is aboujt 3 moles protons per mole ATP. if Delta G = 50 kj/mol for ATP synthesis in mitochondria ,then by hess's law Delta G =50+3(-21.5)= -3.4 KJ/mol and the process of synthesis of ATP at the expenseof the protron gvradient is sponteneous under mitocondrial condition.    (1 ATP /4H+)(10 H+/electron pair)=2.5 ATP/ELECTRON PAIR

ONLY 6 protons are pumped for each electron pair passed from FADH2 to O2 SO ATP yeiled

(1ATP/4H+)(10 H+/electron pair)=1.5 ATP/electron pair

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