Sapling LearningWWCCCRCRNTOR (restriction enzymes) cleave DNA. The The following

Question

Sapling LearningWWCCCRCRNTOR (restriction enzymes) cleave DNA. The The following table shows where different restriction endonucleases abbreviation R represents the (adenine and guanine). The pyrimidines (cytósine, thymine, and abbreviation W represents adenine or thymine uracil) are abbreviated as Y. The Enzyme Target sequenceCleavege EcoRI 5' G 3 CTTAA AATTC 3 G 5' 3' CTTAAG 5 5 GAT ATC 3 3 CTA TAG 5 EcoRV5 GATATC 3 Haelll Hindlll PpuMI 3 CTATAG 5 5 GGCC 3 3' CCGG 5 5 AAGCTT 3 5 A AGCTT 3 3 TTCGAA 5 A 5 GWCCY 3 Which restriction endonucleasefs) would cleave a DNA molecule at the following sequences? b) 5 GATATCCTTCGGCCA 3 a) 5 GAAGATATCCTCGC 3 Hindill Haell Hindi', Haell EcoR co continued below. EcoRI EcoRV Plasmid DNA an the sticky end of the nene in the 5 to 3 directinn gene of interest are cut with the enzyme PpuMI. Write a possible sequence of bases for ,Up & View Solulien O Check Answer") Neot Exit

Explanation / Answer

a) 5'-GAAGATATCCTCGC-3' has one restriction site for ECoRV

b) 5'- GATATCCTTCGGCCA-3' has two restriction sites for ECoRV and HaeIII

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