1. In DNA replication, the double-stranded DNA is unwound by the enzyme helicase

Question

1. In DNA replication, the double-stranded DNA is unwound by the enzyme helicase. In PCR, the unwinding of the DNA can be accomplished by

d) All the above are involved in the unwinding of DNA during PCR.

2. In PCR, primers complementary to specific sequences in the DNA template are added to the reaction mix. These annealed primers are extended by the Taq DNA polymerase.  What is the ultimate fate of the synthetic PCR primers in the amplified DNA?

d) They can be found incorporated into the amplified DNA fragments at their 5' ends.

3. A group of students was trying to amplify a specific DNA fragment using PCR. They set up their reaction and returned the next day to isolate their amplified PCR fragment. Further analysis revealed that their experiment had failed and they had no DNA fragments. What mistakes could they possibly have made? (More than one answer can be correct.)

Explanation / Answer

Ans to Q1:

In DNA replication, the double-stranded DNA is unwound by the enzyme helicase. In PCR, the unwinding of the DNA can be accomplished by?

-- In Polymerase chain reaction the two DNA strands are separated by applying heat at ~96 degree celcius. Thus correct choice:

a) heating the sample to 96 degrees C.

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