BIO 440-01 2018 Electrophoresis Lab Report Cell Biology Laboratory Test 1 March
ID: 207442 • Letter: B
Question
BIO 440-01 2018 Electrophoresis Lab Report Cell Biology Laboratory Test 1 March 8, 2018 Multiple Choice Questions Dr. R. M. Kafoury Select your answers carefully and mark your selection well. There is only one answer that best addresses the question. Total 60 possible points (4 points for each question) 1. The cell biology laboratory is aimed at: a. Studying cell function b. Determining the bio-molecules that may regulate cell function c. Providing hands-on experience in molecular and cellular techniques to study cellular mechanisms and regulation d. All of the above 2. In preparing total protein standards, the following is used: a. Bovine serum albumin (BSA) b. Fetal bovine serum (FBS) c. Platelet derived growth factor d. All of the above 3. Standard calibration curves are prepared by: a. Extracting total cellular proteins b. Preparing known concentrations of the target protein and standardizing the response of the instrument c. Performing western blots d. All of the above 4. ELISA is characterized by being: a. Relatively inexpensive b. A good screening tool c. Time efficient d. All of the above 5. Measurement of protein concentrations in ELISA are achieved by: a. Measuring the molecular weights of target proteins b. Measuring the distance proteins travel on a gel c. Measuring the intensity of color resulting from the reaction of the enzyme attached to the antibody complex with the substrate d. Al of the aboveExplanation / Answer
1) option D. cell bio lab aims to study cell function, determine the biomolecule that regulates cell function, and hands-on experience in mol bio and cell bio techniques.
2) option A. BSA protein is used as a standard for estimation of protein.
3) option B. preparing known concentrations of the targeted protein and standardizing the response of the instrument.
4) option d. rrelatively inexpensive, good screening tool and time efficient.
5) option C. measuring the intensity of color resulting from the reaction of the enzyme attached to the antibody complex with the substrate.
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