Question 1 A Neurospora crassa strain unable to synthesize adenine, ad-3A, and u

Question

Question 1 A Neurospora crassa strain unable to synthesize adenine, ad-3A, and unable to thiamine, thi, was crossed ascospore arrangements. The ad-3A allele is indicated first, the thi allele second. A"+"indicates the phenotype for that allele; a ""indicates the mutant allele. ize the vitamin with a wild-type strain. Analysis of 155 asci revealed the foll 13 45 12 A. Are the two genes linked? Explain your answer. B. If the genes are linked, what is the map distance between them? Show your formula and C. What is the gene centromere distance for each gene? Show your formula and calculations. D. Are the genes, if linked, on the same or different sides of the centromere? Please diagram your answer by making a genetic map. Include all the information that you have obtained in constructing the map.

Explanation / Answer

A) No, the genes are unlinked as the recombinants are formed. Crossing over results in the recombinants with recombination frequencies 0% to 50%. 0% recombination denotes complete linkage adn no recombinants formation; 50% recombination denotes incomplete linkage, the genes are either on different chromosomes or so far apart on the same chromosome and formation of recombinants.

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