In our lab prep space, we have the following solutions and powders: Sodium citra

ID: 207597 • Letter: I

Question

In our lab prep space, we have the following solutions and powders:

Sodium citrate (FW = 43.7 g/mol)

SDS powder (FW = 288.38 g/mol)

0.5 M EDTA

IPTG powder (FW = 238.3 g/mol)

20 mg/mL X-gal solution

1000X ONPG

Premade LB

Premade LB agar

0.5 mM Colchicine

50X gel running buffer

Agarose powder

95% ethanol

100 mg/mL Ampicillin

10X BamHI restriction enzyme digestion buffer

milliQ water

dimethylformamide (DMF)

Please Show Work Typed!

19) (B-gal) How would you make 5 mL of 0.013 M o-nitrophenol-b-D-galactoside (ONPG)?

20) (DNA isolation) How would you make 250 mL of 0. 1 M EDTA solution?

19.
MW of IPTG = 238.3 g/mol
M = 0.013
V = 5 mL

M = (W/MW)(1000/V)
W = (M X MW X V)/1000
= (0.013 X 238.3 X 5)/1000
= 0.01548 g
= 1.5 mg
Weigh 1.5 mg of IPTG and dissolve it in 5 mL of water.

20.
Stock = 0.5 M
Required concentration = 0.1 M
Required volume = 250 mL

C1V1 = C2V2
V1 = (C2V2/C1)
= (0.1 X 250)/0.5
= 50 mL
Take 50 mL of 0.5 M EDTA stoack and add 400 mL of water.

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