8. You are in lab and are given two mutant bacteria lines. One line has a mutati

Question

8. You are in lab and are given two mutant bacteria lines. One line has a mutation in a regulatory protein and i s called tap1-. The second line has a mutation in a cis regulatory site and is called cis1-. The tap1 and cis1 regulatory elements regulate expression of the bac operon. The bac operon consists of two genes: Bac1 and Bac2. A. If tap1 and cis1 regulate gene expression of the bac operon through a positive regulatory mechanism, what gene activity (constitutive, inducible or uninducible) do you predict for Bac1 and Bac2 given the merodiploid analysis of the two strains below i. F' tap1+ cis1- bac1- bac2+/ tap1- cis1+ bac1+ bac2- Bac1: Bac2: i. F tap1 cis1- bac1- bac2+ / tap1+ cisi+ bact+ bac2- Bac1: Bac2 B. If tap1 and cis1 regulate gene expression of the bac operon through a mechanism, what gene activity (constitutive negative rep inducible or uninducible) do you predict for Bact and Bac2 given the

Explanation / Answer

Bac1: inducible

Bac2:uninducible

2. tap1-cis1-bac1-bac2+/tap1+cis1+bac1+bac2-

Bac1:inducible

Bac2:uninducible

Bac1: uninducible

Bac2:constitutive

2. tap1-cis1-bac1-bac2+/tap1+cis1+bac1+bac2-

Bac1:uninducible

Bac2:constitutive

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