To a sample of a protein hydrosylate, a chemist added 1.00 mg of tryptophan, whi

Question

To a sample of a protein hydrosylate, a chemist added 1.00 mg of tryptophan, which was labeled with ^14 C and exhibited a counting rate of 584 cpm above background. After this labeled compound was thoroughly mixed with the samples the mixture was passed through an ion-exchange column. The fraction of eluent containing only tryptophan was collected, and from it an 18.0 mg sample of pure tryptophan was isolated. The isolated sample had a count of 204 cpm in the same detector. What was the weight of tryptophan in the original sample?

Explanation / Answer

labeled tryptohane wirght - 1mg

counts         - 584 CPM

wieght of sample tryptophane - 18mg

C-14 count - 204 CPM

part labeled tryptophane in the sample = 204/504

1mg of iabeled tryptophane is added to the sample

weight of tryptophane in the sample = 18* 504/204 = 44.47 mgm

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