2.(10) An enzyme is inhibited by a small molecule drug. The data from the kineti

Question

2.(10) An enzyme is inhibited by a small molecule drug. The data from the kinetic analysis has been plotted in the form of Lineweaver-Burk plots in the absence and presence of increasing concentrations of drug. Answer the following questions using the plot and the data given on the next page. a) (2 marks) What type of inhibitor does the drug seem to be? Hint: despite appearances the lines do not intersect at the X-axis. b) (2 marks) Which equation describes the initial rate in the presence of the inhibitor? e) 3 marks) In the absence of inhibitor (triangles) linear regression analysis results in a best fit for y = 0.6x + 0.0182, what are the KM and Vmax for the uninhibited enzyme? d) (3 marks) For the inhibited curve (center line, squares) the inhibitor concentration was 80 The regression analysis provides y = 1.08x + 0.0364, what is/are the K value(s) for the drug? Hint: Depending on your answer in part a) there can be one or two Ki values!

Explanation / Answer

a) all the line shows non-competitive type of drug as Vmax is decreased and km remains constant.

b)

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