The image represents the hybridization of a mature eukaryotic mRNA molecule with
ID: 208731 • Letter: T
Question
The image represents the hybridization of a mature eukaryotic mRNA molecule with the corresponding coding region of single stranded DNA (the template strand). A loop has been identified. Select the best answer for the loop.You may want to wait for lecture to answer this question A. The loop represents the entire single strand of DNA, as the mRNA is complementary to only one strand The loop represents the mRNA that is NOT complementary to any portion of the DNA. C. The loop represents single stranded DNA that corresponds to the intron of the mRNA. The loop represents single stranded DNA that corresponds to the exon of the mRNA. E. The loop represents single stranded mRNAExplanation / Answer
1.
Incorrect: This is true that loop represent the entire single stranded DNA. mRNA is also complementary to the DNA template strand but both the strands act as a template to code the correct protein. The mRNA do not code in the loops.
The loop is not the mRNA as this is the part of non coding DNA. Exon is the coding region and loop cannot contain the coding region as it has to remove.
Hence, the options (A), (B), (D), and (E) are incorrect.
Correct: The loop in the diagram is the single stranded DNA, as it is acting as the template strand. This loop corresponds to the intron of the mRNA. DNA hybrids with the mRNA except in the intron regions where the complementary RNA has been removed from the mRNA forming DNA loops in between exons.
Hence, the option (C) is correct.
2.
A. This is true that prior to RNA processing the pre-mRNA would contain introns and exons. The introns are removed with the help of a process called as splicing before the protein synthesis.
B. The given statement is false. This is because poly A tail has been added to the pre-mRNA during the process of splicing.
C. The statement is true that fully mature mRNA would contain a promoter.
D. The given statement is true that fully mature mRNA would contain a ribosome binding site.
E. This statement is also true that fully mature mRNA would contain a 5’ modified end.
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