1. Obeain 3 eest rubes from the instructor pH 4, pH 7, and pH 10. You will perfo
ID: 209302 • Letter: 1
Question
1. Obeain 3 eest rubes from the instructor pH 4, pH 7, and pH 10. You will perform each 2. Add 3 mL of the starch solution to each tube, and mix well. 3. Prepare your spot plate by adding a drop of lodine to each well. Remember to set up the 4. Add I mL of the amylase solution to the tubes mix well. 5. At 15 second intervals (for 2 minutes or until starch is no longer detected), take 1 of the pH tests one at a time. I as a control drop from the tube and test for the presence of starch as in previous exercises. Use the following table to keep track of the results for each test by placing a or - to indicate the presence or absence of starch. pH of buffer 15 sec. | 30 sec. | 45 sec. | 1 min. l min· 2 min. 1 min. 15 sec. 30 sec. 45 sec. min. 10 Note the time it takes for the hydrolysis to reach completion at each pH from the table above and fill in the table below. 6. pH of buffer Time to complete hydrolysis quer bnki doun 10 Were your results what you had predicted before starting the experiment? Why or why not?Explanation / Answer
No, we don’t have the results what we predicted before starting the experiment. As we knew, amylase works very well at a pH 7. The activity of this enzyme will be lower anything below or above this optimum pH. Amylase can work in the range of pH 3 to pH 11. In the observation of this experiment, we have seen that the inactivity of amylase at pH 7. These results are not desirable. At pH 7, the enzyme should work very well and breaks down all the starch. But in this observation, it does not show this activity.
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