the AncA mu- recom- point of view to occur. 5. Brucellosis, point oi, caused by
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the AncA mu- recom- point of view to occur. 5. Brucellosis, point oi, caused by gram-negative Brucelle b disease with serious im pendent ation cies tock industaes and sterility in males. In hu mental design cies, is a zoonotic dise In animals, brucells can (ie, how occurred to sion s is rarein) disease that can be debilita fatal, but it does cause tion ing) disease that can be debi variation Brucella bacteria enter macrophages bu ab reable to s evade phagolysosomal fusion and so a hether recombina- vive intraceliularly. Brucella, unlike distinguish negative bacteria, such as E. coli, has d the out- that does not activate the innate immune ble contriComparisor sequenced genomes of several pon of the strains that are nonpathogenic to humans B riment on a that have (B. meliten nd B. ovis) to those that are pathogenic to humans sis, B. abortus, and B. suis) has revealed that cases, Brucella species are highly conserved overall (ia share 98 to 100% sequence identity at the thegenes nucleotide level, with a G+C% of 573), exceptfor tain distinct regions, called genomic islands (GIs).A group of researchers hypothesized that host specifici and virulence differences must stem from the limited genome diversity found in five of these regions are present in all pathogenic strains: GI-1 (8.1 kb; 9 open reading frames [ORFs]; G+C% 53.2), GI-2 (15.1 kb; 20 ORFs; G+C% = 51.3), GI-3 (21 kb:30 ORFs; G+C% 52.3), GI-5 (44.1 kb:42 ORFs G+C% -57.2), and GI-6 (7.5 kb; 10 ORFs; G+C%-54.2). Comparing these Gls among the spe ared. In but at a n. Also, rain was ty low-iron in high- uld you ght,the that on of ium to cies to that of the e bac- human pathogen B. melitensis, the researchers found well- te hyro- rium GI-2. Based dis- that GI-6 is absent in B. neotomae and GI-1, G1-2, and GI-5 are absent in B. ovis. Below is a schematicdi gram of the the DNA database, the researchers were genetic organization of the ORFs within on sequence homology to other genes in able to an- ell notate some of the ORFs present. The arrowsmra nin th e direction of transcription. OMP, outer on protein; Tnpase, transposase.Explanation / Answer
Open reading frames (ORFs) are parts of a perusing outline that contain no stop codons. A reading frame is a succession of nucleotide triplets that are perused as codons indicating amino acids; a solitary strand of DNA arrangement has three conceivable perusing outlines. Long ORFs may show applicant protein coding areas in a DNA grouping. Districts of DNA that encode proteins are first interpreted into detachment RNA and afterward converted into protein. By inspecting the DNA succession alone we can decide the arrangement of amino acids that will show up in the last protein. In interpretation codons of three nucleotides figure out which amino corrosive will be included next in the developing protein chain. It is vital at that point to choose which nucleotide to begin interpretation, and when to stop, this is called an open reading frames.
In the article provided it is clear that the GC percentage count of different frame is provided and we know that higher the GC percentage more is the chance of inheritance of character as the GC content high means conserved region that is there is no chance of any type of mutation in the particular region.
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